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KMID : 1059519920360040504
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Journal of the Korean Chemical Society
1992 Volume.36 No. 4 p.504 ~ p.510
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Metachromasy of Methylene Blue on the Bacteriorhodopsin Incorporated into L-α-lecithin Vesicle
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Lee Hong
Pae Hyun-Ock
Lim Chun-Ock
Lee Hoo-Seol
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Abstract
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Absorptioon properties of methylene blue (MB) in L-α-lecithin vesicle, bacteriorhodopsin and incorporated bacteriorhodopsin (InBR) vesicle systems at 20∼60℃ has been studied by adsorption spectroscopy. The equilibrium of MB between monomer and dimer in lecithin vesicles has been existed at low concentration of MB, but oligomer has been formed in vesicle at higher concentration of MB. In most cases, the MB cluster was redistributed to monomer at the concentration of lecithin vesicles. Adding BR to constanr concentration of MB deceased the adsorption ratio (α/β) of MB, and MB was formed oligomeric aggregate. abosorption ratio (α/β) of MB was increased during phase transition of InBR vesicles, but independent of phase transition of lecithin vesicles. It suggested that aggregate of MB on the surfaces of InBR vesicles were redistributed to monomer under the influence of lipid phase transition.
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KEYWORD
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